Azide-glucose broth is very nutritious and offers very good growth conditions. The sodium azide contained in it serves as an inhibitor of gram-negative bacteria.Azide Glucose Broth is used to study water and waste water for fecal contamination. The presence of enterococci is considered an indicator of contamination, as they have a relatively high tolerance to chlorine compared to Escherichia coli. The contained sodium azide suppresses the gram-negative accompanying flora of the enterococci.