Brilliantgrün-Phenolrot-Agar n. Edel-Kampelmacher (BPLS)

Abbreviazione: BPLS
Numero articolo: 40-1110
Scheda: Petri Dish, 90mm
Colore: Reddish-brown
Condizioni di stoccaggio prodotti: Dry, in closed bag at 4 – 10°C.
Data di scadenza: 3 Months
Valore pH: 7.0 ± 0.2 at 25°C
Destinazione e applicazione

Modified Brilliant Green Phenol Red Agar according to Edel and Kampelmacher is a selective medium for the isolation of Salmonella from foods. The medium has been modified by increasing the Brilliant Green concentration so that it shows good growth of Gram Positive Bacteria, whereas most Gram Negative bacteria are strongly inhibited.

Composizione tipica

in g per 1 litre of Nutrient medium
Beef Extract5.0
Yeast Extract3.0
Disodium dihydrogen phosphate1.0
Sodium dihydrogen phosphate0.6
Brillant Green0.005
Phenol Red0.09
*Adjusted as required to meet performance standards

Controlli di qualita' microbiologici

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.

Incubation conditions: 24±3 hoursa at 37±1°C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica ssp. abonyNCTC 6017/WDCM 0002950–130 %Pink colonies

Incubation conditions: 24±3 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433/WDCM 00009Complete inhibition-
Escherichia coliATCC 6538/WDCM 00012Growth and partial inhibitionYellow colonies

Mikrobial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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