Caseinpepton-Sojamehlpepton-Agar mit LTH (TSA-LTH)

Recommended by the Harmonised European Pharmacopoeia, supplemented with Lecithin, Polysorbate 80 (Tween 80) and L-Histidine

Abbreviazione: TSA-LTH
Numero articolo: 40-1531
Scheda: Petri Dish, 90mm
Colore: Yellowish
Condizioni di stoccaggio prodotti: Dry, in closed bag at 15 – 22°C.
Data di scadenza: 6 Months
Valore pH: 7.3 ± 0.2 at 25°C
1531 caseinpepton sojamehlpepton agar mit enthemmer tsa lth xebios
Destinazione e applicazione

Tryptone Soya Agar is used for the microbiological examination of non-sterile products. It contains two peptones, which support the growth of a wide variety of organisms. It is suitable for the cultivation of both aerobic and anaerobic bacteria. The later is grown either in deep cultures or by incubation under anaerobic conditions as well as yeasts and moulds.

The high number of microorganisms detectable using Tryptone Soya Agar is due to the addition of peptones gained from enzumatic hydrolysis of casein protein and soya proteins. It includes: the detection of Listeria spp, Pasteurella spp, Vibrio spp, Haemophilus vaginalis or Candida spp.

Tryptone Soya Agar contains no carbohydrates, so it can be used in the investigation of haemolytic reactions. The Pharmacopeia European (PhEur) recommends TSA for the enumeration of Total Viable Count in products under examination for microbial load.

The addition of Lecithin, Tween 80 and Histidine components neutralise the inhibotory effects of aldehydes, phenols, Hexachlorophene, chlorhexidine, formaldehyde and quarternary ammonium compounds used for preservatives, cleaning and disinfection agents.

Composizione tipica

in g per 1 litre of Nutrient medium
Pancreatic-digest of Casein Peptone15.0
Enzyme-digest of Soya Bean Pepton5.0
Sodium Chloride 5.0
Lecithin 0.7
Tween 80 5.0
L-Histidine 0.5
*Adjusted as required to meet performance standards

Controlli di qualita' microbiologici

The Microbiological Performance Test is carried out in accordance with the requirements of PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).

Incubation conditions: 2–3 days at 30–35°C; Inoculum concentration: 10–100 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 6538 / WDCM 0003250–200 %Medium, slightly yellowish colonies
Pseudomonas aeruginosaATCC 9027 / WDCM 0002650–200 %Medium, slightly yellowish colonies
Bacillus subtilisATCC 6633 / WDCM 0000350–200 %Large, flat, dry, irregularly shaped colonies
Candida albicansATCC 10231 / WDCM 0005450–200 %Small, white, dry colonies
Aspergillus brasiliensisATCC 16404 / WDCM 0005350–200 %Dark brown to black conidia on light mycelium
Candida albicans
(3–5 days at 20–25°C)
ATCC 10231 / WDCM 0005450–200 %Small, white, dry colonies
Aspergillus brasiliensis
(3–5 days at 20–25 °C)
ATCC 16404 / WDCM 0005350 – 200 %Dark brown to black conidia on light mycelium

Microbial contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35 °C

No growth present

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