CHROMedium Salmonella/XLD-Agar (ISO)
| Abbreviazione: | CXSA / XLD |
| Numero articolo: | 42-1552 |
| Scheda: | Double Petri dish, 90mm |
| Colore: | Amber color / Red |
| Condizioni di stoccaggio prodotti: | Dry and closed at 15-22°C |
| Data di scadenza: | 4 Weeks |
| Valore pH: | 7.2 ± 0.2/7.4 ± 0.2 at 25°C |
Double plate for the detection and identification of Salmonella
Chrom-X-Salmonella-Ident-Agar:
| in g per 1l Medium | |
|---|---|
| Casein peptone | 5 |
| Beef meat extract | 5 |
| Sodium citrate | 8.5 |
| Chromogenic Mixture | 5.81 |
| Antibiotic Mixture (Mix growth, in mg) | 17 |
| Agar | 13 |
Xylose-Lysine-Deoxycholate Agar nach EP/USP/JP:
| in g per 1l Medium | |
|---|---|
| Yeast extract | 3.0 |
| L-Lysine | 5.0 |
| Xylose | 3.5 |
| Lactose-Monohydrate | 7.5 |
| Sucrose | 7.5 |
| Sodium deoxycholate | 2.5 |
| Sodium thiosulphate | 6.8 |
| Ammonium Iron (III)-citrate | 0.8 |
| Phenol red | 0.08 |
| Sodium chloride | 5.0 |
| Agar | 13.5 |
| *Adjusted as required to meet performance standards |
The microbial performancetest is carried out in conformity with the requirements of DIN EN ISO 11133
Chrom-X-Salmonella-Ident-Agar:
Productivity
Incubation conditions: 24 ± 3 Stunden bei 37 ± 1 °C; Inoculum concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica serovar Enteritidis | ATCC 13076 / WDCM 00030 | 50 – 130 % | Magenta colonies |
Selectivity
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 25922 / WDCM 00013 | Partial inhibition | Blue green colonies | |
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | complete inhibition | - |
MicrobialContamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
Specification| No microbial contamination |
Xylose-Lysine-Deoxycholate Agar nach EP/USP/JP:
Themicrobial performance test is carried out in conformity with the requirementsof DIN EN ISO 11133 and the Pharm. Eur. (Microbiological testing of non-sterileproducts according to Chapter 2.6.13).
Produktivität
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 10 – 100 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica serovar Abony | NCTC 6017 / WDCM 00029 | Good growth | Colonies with a black center and a slightly transparent reddish zone due to the color change of the medium | |
| Salmonella enterica serovar Enteritidis | ATCC 13076 / WDCM 00030 | 50 – 130 % | Colonies with a black center and a slightly transparent reddish zone due to the color change of the medium |
Selectivity
Incubation conditions: 24 ± 3 hours at 37 ± 1 °C; Inoculum concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | complete inhibition | - | |
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | complete inhibition | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| No microbial contamination |