Violet Red Bile Dextrose Agar (VRBD) (VRBD-ISO)

According to ISO 21528


Abbreviazione: VRBD-ISO
Numero articolo: 40-1148
Scheda: Petri Dish, 90mm
Colore: Violet, transparent
Condizioni di stoccaggio prodotti: Dry, in closed bag at 4 – 10°C.
Data di scadenza: 6 Months
Valore pH: 7.4 ± 0.2 at 25°C
1148 kristallviolett neutralrot galle glucose agar vrbd iso xebios
Destinazione e applicazione

Violet Red Bile Dextrose Agar (VRBD) medium is for the detection and enumeration of bile-tolerant gram negative bacteria from foods such as milk and dairy products, egg products and animal carcasses. Moreover VRBD Agar is used for the detection of Enterobacterieceae in meat and dairy products.


Composizione tipica

in g per 1 Litre nutrient medium
Yeast Extract3
Pancreatic-digest of gelatine peptone7
Bile salts1.5
Sodium chloride5
Glucose monohydrate10
Neutral red0.03
Crystal Violet0.002
Agar15
*Adjusted as required to meet performance standards


Controlli di qualita' microbiologici

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.


Productivity
Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: Target Organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica ssp. AbonyNCTC 6017/WDCM 0002950–130 %Pinkish red colonies with no odour or precipitate
Escherichia coliATCC 8739/WDCM 0001250–130 %Pinkish red colonies with no odour or precipitate


Selectivity
Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433/WDCM 00009Complete inhibition-


Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

Specification
No microbial contamination



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