Kristallviolett-Neutralrot-Galle-Glucose-Agar VRBD (VRBD)

Recommended by the Harmonised European Pharmacopoeia

Abbreviazione: VRBD
Numero articolo: 40-1042
Scheda: Petri Dish, 90mm
Colore: Violet, transparent
Condizioni di stoccaggio prodotti: Dry, in closed bag, at 4 – 10°C.
Data di scadenza: 6 Months
Valore pH: 7.4 ± 0.2 at 25°C
Destinazione e applicazione

Violet Red Bile Dextrose Agar (VRBD) medium is used for the detection and enumeration of bile-tolerant gram negative bacteria from foods such as milk and dairy products, egg products and animal carcasses. Moreover VRBD Agar is used for the detection of Enterobacterieceae in meat and dairy products. The culture medium complies with the requirements of the method included in the EP/USP/JP.

Composizione tipica

in g per 1 litre Nutrient medium
Yeast Extract3
Pancreatic-digest of Gelatine Peptone7
Bile Salts/td>1.5
Sodium Chloride5
Glucose Monohydrate10
Neutral Red0.03
Crystal Violet0.002
*Adjusted as required to meet performance standards

Controlli di qualita' microbiologici

The Microbiological Performance Test is carried out in accordance with the requirements of PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).

Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: Targer organisms: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Salmonella enterica ssp. AbonyNCTC 6017/WDCM 0002950–130 %Pink-red colonies and no odour and no precipitate
Escherichia coliATCC 8739/WDCM 0001250–130 %Pink-red coloniesand no odour and no precipitate

Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433/WDCM 00009Complete inhibition-

Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C

No microbial contamination

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