Lactose-TTC-Agar mit Tergitol-7 (LTTC)

Abbreviazione: LTTC
Numero articolo: 40-1278
Scheda: Petri Dish, 90mm
Colore: Blue-green
Condizioni di stoccaggio prodotti: Dry, in closed bag at 4-10°C
Data di scadenza: 3 Months
Valore pH: 7.1 ± 0.2 at 25°C
Destinazione e applicazione

Lactose TTC Agar with Tergitol-7 is a selective and differential medium suitable for the isolation, enumeration and identification of Coliform bacteria in food and water samples. Tergitol-7 is added to this medium to inhibit the growth of Gram-Positive bacteria and to suppress the swarming of Proteus species.

Production of acid from the growth of Lactose-fermenting Coliform bacteria results in the colour of the Bromothymol Blue Indicator in the agar to turn yellow and an insoluble red substance formed by the reduction of Triphenyl-tetrazolium Chloride (TTC) by the coliform bacteria.

Composizione tipica

in g per 1 litre of Nutrient medium
Meat Extract5
Yeast Extract6
Bromthymol Blue0.05
2,3,5-Tri-phenyltetrazolium Chloride (TTC)0.025
*Adjusted as required to meet performance standards

Controlli di qualita' microbiologici

The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).


Incubation conditions: 21 ± 3 Hours at 36 ± 2 °C; Incubation Conditions: 80 – 120 KBE

OrganismType StrainSpecificationColony morphology
Escherichia coliNCTC 13167 / WDCM 0017950 – 130 %Typical, yellow colonies with yellow background
Enterobacter aerogenesATCC 13048 / WDCM 0017550 – 130 %Orange-Yellow colonies
Citrobacter freundiiATCC 43864 / WDCM 0000650 – 130 %Orange-Yellow colonies


Incubation conditions: 21 ± 3 hours at 36 ± 2 °C; Inoculation concentration: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Enterococcus faecalisATCC 19433 / WDCM 00009Complete inhibition-


Incubation conditions: 21 ± 3 hours at 36 ± 2 °C; Inoculation concentration: 1.000 – 10.000 CFU

OrganismType StrainSpecificationColony morphology
Pseudomonas aeruginosaATCC 27853 / WDCM 00025Good growthRed colonies with blue background

Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C

No microbial contamination

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