m-CP-Agar
| Abbreviazione: | MCP |
| Numero articolo: | 45-1288 |
| Scheda: | Petri Dish, 70mm |
| Colore: | Violett, transparent |
| Condizioni di stoccaggio prodotti: | Dry, in closed bag, at 4-10°C |
| Data di scadenza: | 2 Months |
| Valore pH: | 7.4 ± 0.2 bei 25°C |
MCP Agar (Membrane-Clostridium-Perfringens-Agar) is used for the detection and enumeration of Clostridium perfringens (including spores) in water.
| in g per 1 litre of nutrient medium | |
|---|---|
| Tryptose | 30 |
| Yeast Extract | 20 |
| Saccharose | 5 |
| L-Cysteine Hydrochloride | 1 |
| Magnesium Sulphate, anhydrous | 0.048 |
| Indoxyl-ß-Glucoside | 0.06 |
| Bromocresol Purple | 0.04 |
| Phenolphthalein-Diphosphate | 0.1 |
| Ferric (III)Chloride | 0.09 |
| Cycloserine | 0.4 |
| Polymyxin B-Sulfat | 0.025 |
| Agar | 12.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and PhEur. (Microbiological Examination of Non-Sterile Products in accordance with Chapter 2.6.13).
Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculum concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Clostridium perfringens | ATCC 13124 / WDCM 00007 | 50 – 130 % | Yellow colonies; Phosphatase Test Positive |
Selectivity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculum concentration: 10,000-1,000,000 CFU, anaerobic incubation
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - |
Specificity
Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculation concentration: 1.000-10.000 CFU, anaerobic incubation
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Clostridium bifermentans | NCTC 506 / WDCM 00079 | - | Blue colonies; Phosphatase Test Negative |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C
| Specification |
|---|
| No microbial contamination |