Pseudomonas-CN-Agar (PCN)
According to ISO 16266
| Abbreviazione: | PCN |
| Numero articolo: | 40-1286 |
| Scheda: | Petri Dish, 90mm |
| Colore: | transparent |
| Condizioni di stoccaggio prodotti: | Dry, in closed bag at 4 – 10°C. |
| Data di scadenza: | 3 Months |
| Valore pH: | 6.8 ± 0.2 at 25°C |
Pseudomas CN Agar Selective Agar for the cultivation of Pseudomonas aeruginosa with the addition of Cetrimide to suppress the growth of non-target bacteria, such as Klebsiella, Proteus or other prominent species in water.
| in g per 1 litre of Medium | |
|---|---|
| Gelatin Peptone | 16.0 |
| Casein Hydrolysate | 10.0 |
| Potassium Sulphate, anhydrous | 10.0 |
| Magnesium Chloride, anhydrous | 1.4 |
| Agar | 11.0 |
| Cetrimide (in mg) | 200.0 |
| Nalidixic Acid (in mg) | 15.0 |
| Glycerine (in ml) | 10.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133:2014.
Productivity
Incubation conditions: 2–3 days at 30–35 °C; Inoculation concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Pseudomonas aeruginosa | ATCC 10145 / WDCM 00024 | 50 – 130 % | Blue-Green to greenish colonies with fluorescence under UV light (360 ± 20 nm) |
Selectivity
Incubation conditions: 44 ± 4 Hours at 36 ± 2 °C; Inoculation concentration: 10.000–1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition | - | |
| Escherichia coli | Complete inhibition | - |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25 °C and 5–7 days at 30–35 °C
| Specification |
|---|
| No microbial contamination |