Tryptose-Sulfit-Cycloserin-Agar (TSC-Agar) (TSC)

Abbreviazione: TSC
Numero articolo: 40-1241
Scheda: Petri Dish, 90mm
Colore: Light beige
Condizioni di stoccaggio prodotti: Dry, in closed bag, at 4 – 10°C.
Data di scadenza: 4 Weeks
Valore pH: 7.6 ± 0.2 at 25°C
Destinazione e applicazione

Tryptose-Sulfit-Cycloserin-Agar is used for the presumptive identification and enumeration of Clostridium perfringens and other sulfite-reducing Clostridia in food, water and clinical samples. It contains nutrients to promote the growth of Clostridia and ensure their rapid proliferation. The inclusion of Ammonium-Iron-(III)-Citrate and the Sodium Meta bisulphite are indicators of sulphite reduction by Clostridium perfringens which produces black colonies.

Composizione tipica

in g per 1 litre of nutrient medium
Soya Bean Peptone5.0
Yeast Extract5.0
Sodium Meta bisulphite, anhydrous1.0
Ammonium Iron (III) Citrate1.0
*Adjusted as required to meet performance standards

Controlli di qualita' microbiologici

The Microbiological Performance Test is carried out in accordance with the requirements ISO 11133.

Incubation conditions: 21 ± 3 hours at 44 ± 1 °C; Inoculation concentration 80–120 CFU; Anaerobic incubation.

OrganismType StrainSpecificationColony morphology
Clostridium perfringensATCC 13124 / WDCM 0000750 – 130 %Black colonies

Incubation conditions: 21 ± 3 Hours at 44 ± 1 °C; Inoculum concentration: 10,000 – 1,000,000 CFU; Anaerobic incubation

OrganismType StrainSpecificationColony morphology
Bacillus subtilisATCC 6633 / WDCM 00003Complete inhibition. -

Microbial Contamination
Incubation conditions: 2–7 days at 20–25 °C and 5–7 days at 30–35 °C

No microbial contamination

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